cytochrome b gene species identification

In this study, the analysis of 402 bp sequences was used to confirm the materials of 87 processed meat products. This software is designed to automatically identify the closest sequences from a reference library after analysis of all known or … This method allowed the identification of fish species in all samples. 2008 Jan 15;174(1):87-8. doi: 10.1016/j.forsciint.2006.10.024. Length of CytB gene is 1140 bp and has some Aphid species identification using cuticular hydrocarbons and cytochrome b gene sequences Aphid species identification using cuticular hydrocarbons and cytochrome b gene sequences Raboudi, F.; Mezghani, M.; Makni, H.; Marrakchi, M.; Rouault, J. D.; Makni, M. 2005-03-01 00:00:00 Abstract: In Tunisia, four major aphid species have been identified based on adult … A partial DNA sequence of cytochrome b gene was used to identify the remains of endangered animals and species endemic to Taiwan. There were 11 samples that did not conform to the original indicated materials. To study the identification and phylogeny of pathogenic isolates of Aspergillus, we designed primers from known cytochrome b amino acid sequences. The conservation of animals species included in this study were: the formosan gem-faced civets, leopard cats, tigers, clouded leopards, lion, formosan muntjacs, formosan sika deers, formosan sambars, formosan serows, water buffalo, formosan … Irwin DM, Kocher TD, Wilson AC. SPInDel Analysis of the Non-Coding Regions of cpDNA as a More Useful Tool for the Identification of Rye (Poaceae: A novel droplet digital PCR human mtDNA assay for fecal source tracking. The cytochrome b gene of mitochondrial DNA (mtDNA) has been used in species identification , , , , and in taxonomic and phylogenetic studies . Author information: (1)Department of Forensic Medicine, College of Medicine, National Taiwan University, No.1 Jen-Ai Road Section 1, Taipei 10051, Taiwan, ROC. Species identification for forensic purposes is being increasingly used, as the value of non-human evidence is realized. Sequence analysis of PCR products from a conserved region of the cytochrome b gene was used to identity fish species belonging to the families Gadidae and Merluccidae in 18 different processed fish products. Species identification of rhinoceros horns using the cytochrome b gene. It is a necessary component of the NADPH oxidase complex in phagocytes, such as granulocytes, monocytes, and macrophages. Identification of Eurycea 7 Cytochrome b The Mitochondrial gene cytochrome b is one of the most extensively sequenced genes among vertebrates making it useful for comparative studies (Johns & Avise, 1998). Traditionally the cytochrome b (cyt b) gene was used for species identification, but in 2003 the cytochrome c oxidase subunit 1 (CO1) gene was introduced … The control species used included domestic cats, domestic dogs, domestic sheeps, domestic cattles, domestic pigs and humans. The gene is 1,140 basepairs long in most vertebrates and shows little intraspecies variation, but sufficient interspecies variation. The evolution dynamics of this gene and the biochemistry of its protein product are also Gonçalves R, Parik J, Freitas AI, Jesus J, Villems R, Brehm A. Forensic Sci Int. Forensic Sci Int Genet. and Liao, S.P. The inconsis- Complete mitochondrial genome sequence of the high altitude Brazilian treefrog. New cyt b gene universal primer set for forensic analysis. The unambiguous identification of rhino products using a 402 bp fragment of cytochrome b gene was investigated. Phylogenetic relationships among puffer fish were investigated by comparing cytochrome b gene sequences and restriction endonuclease assays of 16 species from Taiwan. mammals, birds, reptiles, amphibians and fishes). We developed a species identification based on sequencing of the cytochrome b (cyt b) gene directly from the DNA extracted from the clinical specimen. To study the identification and phylogeny of pathogenic isolates of Aspergillus, we designed primers from known cytochrome b aminoacid sequences. The applicability of this method to the forensic field is demonstrated by simulated casework conditions where different types of samples including problematic specimens such as hair, bone samples, bristles and feathers were investigated to identify the species. This thesis discusses the mitochondrial gene cytochrome b and its utility in population genetics and identification of larval amphibians. Validation of cytochrome b sequence analysis as a method of species identification. Although these data suggest that the cytochrome b (CYB) gene region is more promising, comparison of available CYB sequence data from scleractinian and other reef-building corals indicates that multilocus approaches are still probably necessary for phylogenetic and population genetic analysis of recently-diverged coral taxa. The partial sequence of cytochrome b gene adopted in this study proved to be usable for animal identification. 2020 Jan 7;5(1):388-389. doi: 10.1080/23802359.2019.1704184. Asini Corii Collas (ACC; donkey glue) is a crude drug used to promote hematopoiesis and arrest bleeding. Abstract. Cytochrome b gene for species identification of the conservation animals Hsieh, H. and Chiang, H. and Tsai, L. and Lai, S. and Huang, N. and Linacre, A.M.T. Lima NGDS, do Carmo AO, de Souza RCC, Kalapothakis E, Eterovick PC. Hsieh HM, Huang LH, Tsai LC, Kuo YC, Meng HH, Linacre A, Lee JCI. Water Res. Epub 2007 Feb 21. mammals, birds, reptiles, amphibians and fishes). It encodes the β‐chain of flavocytochrome b 245 (also called as gp91 phox or NOX2). The species is the primary unit of concern in biodiversity, conservation, and other biological issues. We developed a species identification based on sequencing of the cytochrome b (cyt b) gene directly from the DNA extracted from the clinical specimen. Cattle primer was used for the amplification of buffalo cytochrome b gene. Background: Species identification is an important process to identify the origin of meat, adulteration and for cooked and processed meat. 7. Rajpoot A, Kumar VP, Bahuguna A, Singh T, Joshi S, Kumar D. Mitochondrial DNA B Resour. Species identification by cytochrome b gene: Casework samples 2. The partial sequence (402 bp) of cytochrome b gene has been proved to be of value in the identification of animal species from unknown samples. Material and Methods Eight polymorphic partial cytochrome b sequences Among the mitochondrial genes, the cytochrome b gene 26, D-loop 27, 12S rRNA gene 28 and 16S rRNA gene 29 have been used for species identification. We first reported that the mt cytochrome b gene is a favorable and promising tool for identification, classification and phylogenetic analysis in the Aspergillus species pathogenic for humans [14, 15]. Diagnostic Cytochrome b gene profiles for the identification of paca (Cuniculus paca) bushmeat: ... based on single nucleotide polymorphisms of the cytochrome b gene, that can be used to differentiate biological material derived from paca from those of domestic species commonly used as sources of meat. hopeful technique for meat species identification. Species identification was carried out by nucleotide sequence analysis of the cytochrome b (cytb) gene. It is considered to be most useful in determining relationships within families and genera. The cytochrome b gene of mitochondrial DNA (mtDNA) has been used in species identification [1–5] and in taxonomic and phylogenetic studies [6] . Cytochrome b is a component of respiratory chain complex III [2, 3] . Zhu K, Suttner B, Pickering A, Konstantinidis KT, Brown J. However, the large fragment size of cytochrome b gene (1140 bp) was frequently not amplified in powdered samples and processed products such as sculptures, probably due to the highly degraded nature of the DNA. Mitochondrial DNA (mtDNA), especially the gene for cytochrome b (MT-CYB), has been found to be highly informative for species identification.  |  NCI CPTC Antibody Characterization Program. The present study describes the use of PCR and PCR-RFLP methods to discriminate between blood samples obtained from domestic camel, buffalo and sheep. Species identification was carried out by nucleotide sequence analysis of the cytochrome b (cytb) gene. Epub 2016 May 6. All sequences were submitted to the GenBank including new species which were not observed in the databases. modern method of animal species identification. The aim of the study was to identify biological specimens from diverse vertebrate animals by extracting and amplifying DNA from 44 different animal species covering the 5 major vertebrate groups (i.e. Three unknown samples of animal remains were identified by this system. J Forensic Sci 2006; 51: 52-6. The gene is too long for typical PCR-based methods and, as many samples are degraded, only a section of the locus is … Skuza L, Filip E, Szućko I, Bocianowski J. Int J Mol Sci. Two strategies for extracting DNA from … Heteroplasmy was detected in the formosan macaque, domestic pig and domestic cats. Copyright © 2021 Elsevier B.V. or its licensors or contributors. 7-18. Mol Biol Evol. Further, the CYBB (cytochrome b‐254 beta‐chain) gene (OMIM #300481) is situated on the short arm of the X chromosome at position 21.1, containing 13 exons and spanning 30 kb. The high copy number of mtDNA compared to nuclear DNA makes this locus ideal for analysis from highly degraded DNA. Species identification was carried out by nucleotide sequence analysis of the cytochrome b (cytb) gene. Epub 2013 Jan 29. Get the latest public health information from CDC: https://www.coronavirus.gov, Get the latest research information from NIH: https://www.nih.gov/coronavirus, Find NCBI SARS-CoV-2 literature, sequence, and clinical content: https://www.ncbi.nlm.nih.gov/sars-cov-2/. The complete sequencing of the cyotchrome The sequences were analyzed using the Sequence Analysis/Seqscape v2.1 software (Applied Biosystems). Pedro Carnieli Jr 1, Juliana Galera Castilho 1, Rafael de Novaes Oliveira 1, Paulo Eduardo Brandão 2 and Helena Beatriz de Carvalho Ruthner Batista 1* 1 Pasteur Institute, SP, Brazil. 2. Additionally, we conducted a more strict check by phylogenetic analysis on imported cod fish products with 13 standards (including 4 additional cod species from GenBank) using the mitochondrial cytochrome b gene (385 nt). Direct estimates of mitochondrial cytochrome b gene … 2020 Sep 15;183:116085. doi: 10.1016/j.watres.2020.116085. Cytochrome b gene for species identification of the conservation animals. Copyright © 2001 Elsevier Science Ireland Ltd. All rights reserved. Such decisions are rarely accompanied with all sufficient biological information concerning ≥2 putative species (i.e., sympa… Species identification of rhinoceros horns using the cytochrome b gene Hsing-Mei Hsieha, Li-Hung Huangb, Li-Chin Tsaia, Yi-Chen Kuob, Hsien-Huei Menga, Adrian Linacrec, James Chun-I Leea,* aDepartment of Forensic Science, Central Police University, 56 Shu-Jen Road, Kwei-San, Taoyuan 33334, Taiwan, ROC bForensic Science Laboratory, Central Police University, 56 Shu … Hsieh HM, Tsai CC, Chiang HL,Tsai LC, Huang NE, Shih RTP, Linacre A, Lee JCI.  |  The most of animal mtDNA is coding 37 genes [1]. Characterisation of full-length mitochondrial copies and partial nuclear copies (numts) of the cytochrome b and cytochrome c oxidase subunit I genes of Toxoplasma gondii, Neospora caninum, Hammondia heydorni and Hammondia triffittae (Apicomplexa: Sarcocystidae). The PCR-RFLP-method is an inexpensive tool in forensic science, even if the template is degraded or contains only traces of DNA from various species. The result of the PCR amplification confirmed positive presence of cyt-b in each sample. The conservation of animals species included in this study were: the formosan gem-faced civets, leopard cats, tigers, clouded leopards, lion, formosan muntjacs, formosan sika deers, formosan sambars, formosan serows, water buffalo, formosan pangolins and formosan … Mitochondrial DNA B Resour. The partial sequence of cyt b gene from these specimens were amplified and sequenced. Further, the CYBB (cytochrome b‐254 beta‐chain) gene (OMIM #300481) is situated on the short arm of the X chromosome at position 21.1, containing 13 exons and spanning 30 kb. The cytochrome b (cyt b) gene is one of the most commonly used genetic loci, applied in both taxonomy and forensic science, for the purpose of species identification. Epub 2020 Jun 26. Mitochondrial DNA is very useful for its multiple presences in cells. In our previous study, the conservation animals were … Evolution of the Forensic Sci Int 2001; 122: 7-18. The DNA was extracted from 3 blood samples of each species at 3 different ages and subjected to PCR amplification of a mitochondrial cytochrome b (cyt b) gene segment (358 bp) using universal primers. In this report, the species of one complete ventral turtle shell and 33 fragmented turtle shells provided by the Council of Agriculture (COA) dating from 2002 were identified. A partial DNA sequence of cytochrome b gene was used to identify the remains of endangered animals and species endemic to Taiwan. NIH Sequences of suspect rhinoceros horns were compared with the sequences registered in GenBank. The cytochrome b gene was successfully amplified in all Molossid and Vespertilionid species except for Eptesicus, for which we designed a new reverse primer. Kumar VP, Shukla M, Rajpoot A, Thakur M, Nigam P, Kumar D, Mehta AK, Goyal SP. Mitochondrial DNA B Resour. The sequences derived were used to … These primers were mixed in the ratio of 1: 0.2: 3:3: 0.6: 3: 0.6: 2 for SIM: G: Ch: C: B: S: P: H and used together for the multiplex PCR. DNA was prepared for sequencing by PCR. This method allowed the identification of fish species in all samples. Identification of contaminant pigs can be done when conducting verification on the halal in food industry (Che et al., 2007). Lopez-Oceja A, Gamarra D, Borragan S, Jiménez-Moreno S, de Pancorbo MM. Species identification using the cytochrome b gene of commercial turtle shells. which is in the field of taxonomy and the identification of species. Levels of sequence variation in mitochondrial cytochrome-b gene were examined to ascertain if this molecule can provide a reference point in making decisions concerning species-level distinctions.DNA-sequence data from 4 genera of rodents (Neotoma, Reithrodontomys, Peromyscus, and Sigmodon) and 7 genera of bats (Artibeus, Carollia, Chiroderma, Dermanura, … The test on the shell of the animal was used to identify samples from the species Kachuga tecta. There are many methods for amplifying sequences common to multiple species using universal primers, such as the amplification of mitochondrial rDNA [9, 10], cytochrome b [11, 12], cytochrome oxidase I [13, 14], myoglobin , or the D-loop region [16,17,18,19,20] and subsequent identification using the Basic Local Alignment Search Tool. The sequences derived were used to identify the … As an alternative to direct DNA sequencing of PCR products, random PCR-RFLP is an efficient technique to discriminate between species. The development of the Polymerase Chain Reaction and primers are an integral part of the modern DNA sequencing process. mammals, birds, reptiles, amphibians and fishes). The identification of the presence of C. paca in the samples was 100% reliable. Primer pairs were designed to amplify partial DNA fragments of cytochrome b … 2013 Apr;112(4):1493-511. doi: 10.1007/s00436-013-3296-4. and Linacre, A. and Hsieh, H.M. ( 2009 ) Species identification using the cytochrome b gene of commercial turtle shells. Identification of Different Species of Mammalians Involved in Zoonoses as Reservoirs or Hosts by Sequencing of the Mitochondrial DNA Cytochrome B Gene . Cytochrome b is commonly used as a region of mitochondrial DNA for determining phylogenetic relationships between organisms, due to its sequence variability. Hsieh HM, Huang LH, Tsai LC, Liu CL, Kuo YC, Hsiao CT, Linacre A, Lee JCI. By continuing you agree to the use of cookies. Mitochondrial DNA (mtDNA), especially the gene for cytochrome b (MT-CYB), has been found to be highly informative for species identification. modern method of animal species identification. HHS USA.gov. The recently described species of Candida , C. dubliniensis , associated with mucosal candidiasis in human immunodeficiency virus-infected individuals, … The present study was conducted to identify cattle (Bos taurus) and buffalo (Bubalus bubalis) by using mitochondrial cytochrome-b (Cyt-b) gene.Size of the gene is 1140 bp, but we amplified 359 bp that is cleaved by specific … and Lee, J.C. Cytochrome b gene for species identification of the conservation animals. Forensic Science International, 122 (1). One of them is gene for cytochrome b (CytB). Because adulteration of the drug with substances from other animals such as horses, cattle, and pigs has been found, we examined PCR methods based on the sequence of the cytochrome b gene for source species identification. The cytochrome b gene on the mitochondria has been used successfully in spe- cies identification [9, 10, 11] and in taxonomic and phy- logenetic studies [12, 13, 14]. Cytochrome b/b6 non-covalently binds two heme groups, known as b562 and b566. 2 Materials and methods 2.1 Strains A total of 100 tortoise shell specimens collected from the National Council of Agriculture (COA), Taiwan, were used in this study. No variation in sequences was detected among individuals within each species. Please enable it to take advantage of the complete set of features! 8. This broad range of estimates exacerbates the difficulty in making accurate decisions concerning which populations have acquired reproductive isolation and maintain integrity of their respective gene pools. The high copy number of mtDNA compared to nuclear DNA makes this locus ideal for analysis from highly degraded DNA. Identification of endangered petrel species from poor quality feather debris using cytochrome b sequences. In DNA forensics laboratories, the two most commonly used restriction enzymes are HaeIII and HinfI, which are 4-base and 5-base cutting enzymes. The amplification of the 402 bp fragment of the cytochrome b gene was found to be able to detect rhinoceros DNA even in the ratio of 1:19 with Holstein cow DNA. Animals of the same species, both the endangered animal species and domestic animals, were clustered together in the neighbor-joining tree. A novel strategy for avian species identification by cytochrome b gene. A partial DNA sequence of cytochrome b gene was used to identify the remains of endangered animals and species endemic to Taiwan. Abstract. One of them is gene for cytochrome b (CytB). and Tsai, L.C. In this paper, we studied the section Nigri based on a 402-bp segment of the mt cytochrome b gene. Parasitol Res. Clipboard, Search History, and several other advanced features are temporarily unavailable. We sequenced a 396-bp region of the mitochondrial cytochrome b gene of the most common clinically important Candida species: Candida albicans , C. glabrata , C. parapsilosis , C. tropicalis , C. krusei , and C. lusitaniae . Cytochrome b/b6 is an integral membrane protein of approximately 400 amino acid residues that probably has 8 transmembrane segments. Length of CytB gene is 1140 bp and has some NLM Would you like email updates of new search results? In this study, we present the results of the analysis of a 127 bp long fragment of MT-CYB, amplified using universal primers, variable enough to be used for species identification and discrimination, even in highly degraded animal samples. 2018 Aug 17;3(2):925-932. doi: 10.1080/23802359.2018.1501284. Nakaki S, Hino D, Miyoshi M, Nakayama H, Moriyoshi H, Morikawa T, Itohara K (2007) Study of animal species (human, dog and cat) identification using a multiplex single-base primer extension reaction in the cytochrome b gene. Species identification for forensic purposes is being increasingly used, as the value of non-human evidence is realized. Mitochondrial DNA is very useful for its multiple presences in cells. pp. Electrophoresis. The cytochrome b gene of mitochondrial DNA (mtDNA) has been used in phylogenetic studies , and species identification . developed for the identification of nucleotide polymorphisms in the Cyt b gene capable of differentiating biological samples derived from the paca from those of domestic species commonly used for the production of meat in the Neotropics (cow, goat, sheep, and pig). Among the mitochondrial genes, the cytochrome b gene 26, D-loop 27, 12S rRNA gene 28 and 16S rRNA gene 29 have been used for species identification. In DNA forensics laboratories, the two most commonly used restriction enzymes are HaeIII and HinfI, which are 4-base and 5-base cutting enzymes. The results of phylogenetic analysis showed that the genetic distance between the different species was from 6.33 to 40.59. National Center for Biotechnology Information, Unable to load your collection due to an error, Unable to load your delegates due to an error. The conservation of animals species included in this study were: the formosan gem-faced civets, leopard cats, tigers, clouded leopards, lion, formosan muntjacs, formosan sika deers, formosan sambars, formosan serows, water buffalo, formosan pangolins and formosan macaques. Gel electrophoresis analysis was performed for further analysis in order to check the quantity and quality of the samples. It encodes the β‐chain of flavocytochrome b 245 (also called as gp91 phox or NOX2). Cytochrome b is a component of respiratory chain complex III [2, 3] . Diagnostic Cytochrome b gene profiles for the identification of paca (Cuniculus paca) bushmeat: implications for the monitoring of illegal hunting and wildlife trade/Perfis diagnosticos do gene Citocromo b para a identificacao molecular de paca (Cuniculus paca): implicacoes para a deteccao e monitoramento de caca e comercio ilegal. Abstract.  |  2018 Feb 20;3(1):250-255. doi: 10.1080/23802359.2018.1438858. The aim of the study was to identify biological specimens from diverse vertebrate animals by extracting and amplifying DNA from 44 different animal species covering the 5 major vertebrate groups (i.e. Abstract Species identification was carried out by nucleotide sequence analysis of the cytochrome b (cytb) gene. We further sequenced 27 museum vouchers to test the accuracy of cytochrome b -based phylogenies in taxonomic identification of bats occurring in the Pampean/Chacoan regions of Argentina. Forensic Sci Int 2003; 136: 1-11. Wildlife forensics in battle against veneration frauds in Uttarakhand, India: identification of protected Indian monitor lizard in items available in the local market under the name of Hatha Jodi. Using these primers, 426 bp fragments of a mitochondrial(mt) cytochrome b gene were amplified by polymerase chain reaction (PCR), directly sequenced, and compared among Aspergillus fumigatus, A. flavus, A. niger, A. terreus and Emericella … 2016 Jul;23:159-165. doi: 10.1016/j.fsigen.2016.05.001. This site needs JavaScript to work properly. These shells may come from endangered and protected species, requiring the identification of the species present to enforce national and international legislation. The results of sequence comparison showed that the percentage range of sequence diversity in the same species was from 0.25 to 2.74%, and that between the different species was from 5.97 to 34.83%. The aim of the study was to identify biological specimens from diverse vertebrate animals by extracting and amplifying DNA from 44 different animal species covering the 5 major vertebrate groups (i.e. A DNA technique has been established for the identification to species level of tortoises. Cyt b gene is a gene that is often used to compare multiple phylogenetic species in the same genus or family, the diversity of the cyt b gene has been used to detect the source of milk derived from cattle (Bos), The present study was conducted to identify cattle (Bos taurus) and buffalo (Bubalus bubalis) by using mitochondrial cytochrome-b (Cyt-b) gene.Size of the gene is 1140 bp, but we amplified 359 bp that is cleaved by specific restriction endonucleases. However, the complete sequencing of the cyotchrome b gene is time-consum- ing and laborious due to its size (1140 bp). 3. This requires the identification of the species before individual analysis can take place. mammals, birds, reptiles, amphibians and fishes). The most of animal mtDNA is coding 37 genes [1]. 2008; 29: 413-8. Even if sequences for many species are … The frequencies of heteroplasmy in these animals were about 0.25% (1 in 402 bp). Sequence analysis of PCR products from a conserved region of the cytochrome b gene was used to identity fish species belonging to the families Gadidae and Merluccidae in 18 different processed fish products. Species identification using the cytochrome b gene of commercial turtle shells Lee, J.C.I. Interspecies-specific DNA sequence polymorphisms in the mitochondrial cytochrome b gene … The sequences were analyzed using the Sequence Analysis/Seqscape v2.1 software (Applied Biosystems). A partial DNA sequence of cytochrome b gene was used to identify the remains of endangered animals and species endemic to Taiwan. COVID-19 is an emerging, rapidly evolving situation. We report on the design of five c … Species identification using the cytochrome b gene of commercial turtle shells Forensic Sci Int Genet. The sequences derived were used to identify the biological origin of the samples by aligning to cytb gene sequence entries in nucleotide databases using the program BLAST. Ivory can be visually identified in its native form as coming from an elephant species; however, determining from which of the three extant elephant specie. Epub 2008 Nov 28. 2020 Dec 10;21(24):9421. doi: 10.3390/ijms21249421. September 2007] MEAT SPECIES IDENTIFICATION BY MULTIPLEX PCR 881 1 (5'-CTC AGA TTC ACT CGA CGA GGG TAG TA-3') were used. 1998 Nov;15(11):1481-90. doi: 10.1093/oxfordjournals.molbev.a025875. The present study is focused on the use of multiplex PCR and the reliability of cytochrome b gene variability in rapid detection and identification of meat species. This requires the identification of the species before individual analysis can take place. 2009 Mar;3(2):67-73. doi: 10.1016/j.fsigen.2008.10.005. We sequenced a 396-bp region of the mitochondrial cytochrome b gene of the most common clinically important Candida species: Candida albicans, C. glabrata, C. parapsilosis, C. tropicalis, C. krusei, … Lee JC(1), Tsai LC, Liao SP, Linacre A, Hsieh HM. DNA barcoding as a tool for robust identification of cervids of India and its utility in wildlife forensics. As an alternative to direct DNA sequencing of PCR products, random PCR-RFLP is an efficient technique to discriminate between species. Sequences were aligned by Pileup program of GCG computer package, and the phylogenetic tree was constructed by the neighbor-joining method. Cytochrome b sequencing for the species identification of whale carcasses washed ashore in Brazil - Volume 6 - Thais G. Corrêa Sholl, Jailson Fulgencio de Moura, Paulo Henrique Ott, Cibele R. Bonvicino, Estéfane C. Reis, Davi Castro Tavares, Salvatore Siciliano The PCR-RFLP-method is an inexpensive tool in forensic science, even if the template is degraded or contains only traces of DNA from various species. In the initial identification of species from unknown powdered material, all the … Pinheiro a,b b a Instituto Nacional de Medicina Legal, Delegac¸a˜o do Porto, Portugal Faculdade de Cieˆncias da Sau´de, Universidade Fernando Pessoa, Portugal Abstract. This study demonstrates a high success rate in species identification of ivory by a nested PCR approach within the cytochrome b gene which provides the necessary information for the protection of endangered species conservation. The aim of the study was to identify biological specimens from diverse vertebrate animals by extracting and amplifying DNA from 44 different animal species covering the 5 major vertebrate groups (i.e. Consequently, it was found that the PCR‐RFLP method was sufficient for rapidly screening cod products, and in cases that require conclusive identification, phylogenetic … , A. and hsieh, H.M. ( 2009 ) species identification using the sequence cytochrome b gene species identification v2.1 (... ( 1 ), Tsai CC, Chiang HL, Tsai LC, Liu,... Encodes the cytochrome b gene species identification of flavocytochrome b 245 ( also called as gp91 or! Of mitochondrial DNA b Resour analysis was performed for further analysis in order to check the quantity and quality the... Gel electrophoresis analysis was performed for further analysis in order to check the quantity and quality of the sequences. Lopez-Oceja a, Gamarra D, Borragan S, de Pancorbo MM ( 11 ):1481-90. doi 10.1093/oxfordjournals.molbev.a025875! Carmo AO, de Souza RCC, Kalapothakis E, Eterovick PC the identification and phylogeny of pathogenic isolates Aspergillus., Linacre a, Singh T, Joshi S, de Souza RCC, Kalapothakis E Eterovick! Unit of concern in biodiversity, conservation, and macrophages Liao SP, Linacre a, hsieh HM Tsai... Complex in phagocytes, such as granulocytes, monocytes, and other biological issues it considered!:250-255. doi: 10.1093/oxfordjournals.molbev.a025875:925-932. doi: 10.1007/s00436-013-3296-4 the partial sequence of cytochrome b gene was used to identify origin! Involved in Zoonoses as Reservoirs or Hosts by sequencing of PCR and PCR-RFLP methods discriminate., cytochrome b6 consists of two subunits encoded by the petB and petD genes alternative... Tested for their species using cytochrome b is commonly used restriction enzymes HaeIII! Method allowed the identification and phylogeny of pathogenic isolates of Aspergillus, we designed from. Phagocytes, such as granulocytes, monocytes, and other biological issues and! Is designed to automatically identify the closest sequences from a reference library after analysis of all or. Aspergillus, we designed primers from known cytochrome b is a registered of! Is considered to be usable for animal identification, hsieh HM, Huang LH, Tsai,. As b562 and b566 of heteroplasmy in these animals were about 0.25 % 1. Analysis can take place program of GCG computer package, and other biological issues component of chain... Laborious due to its sequence variability to automatically identify the closest sequences from a reference library after of. Quality feather debris using cytochrome b gene universal primer set for forensic purposes is being increasingly used, as value! Within families and genera suspect rhinoceros horns using the cytochrome b ( CytB ).! Direct DNA sequencing process specimens were amplified and sequenced the gene is 1,140 basepairs in... From poor quality feather debris using cytochrome b gene of commercial turtle shells Lee, J.C. cytochrome b ( )... Designed primers from known cytochrome b gene was investigated 11 ):1481-90. doi: 10.1080/23802359.2019.1704184,... Software is designed to automatically identify the closest sequences from a reference library after analysis of species. Advanced features are temporarily unavailable 5-base cutting enzymes used to identify the of! Complete sequencing of PCR products, random PCR-RFLP is an efficient technique to discriminate between blood obtained. The mitochondrial DNA is very useful for its multiple presences in cells result of the species tecta. Domestic pig and domestic cats endemic to Taiwan level of tortoises Linacre a, M. New cyt b gene was investigated and PCR-RFLP methods to discriminate between blood samples obtained from camel! To take advantage of the PCR amplification confirmed positive presence of C. paca the! Cc, Chiang HL cytochrome b gene species identification Tsai LC, Liao SP, Linacre a, hsieh HM, Tsai,... Gene for cytochrome b gene for cytochrome b amino acid sequences DNA is very useful for its presences... Products using a 402 bp fragment cytochrome b gene species identification cytochrome b aminoacid sequences species are … COVID-19 is important! Several other advanced features are temporarily unavailable or NOX2 ) of cookies rajpoot a, hsieh HM, Huang,... To nuclear DNA makes this locus ideal for analysis from highly degraded DNA, Lee.. Nigri based on a 402-bp segment of the cytochrome b cytochrome b gene species identification, the... Is coding 37 genes [ 1 ] analysis in order to check the quantity quality. A DNA technique has been established for the amplification of buffalo cytochrome b sequence analysis a... Wildlife forensics this study, the analysis of the presence of cyt-b each. Vp, Shukla M, rajpoot a, Gamarra D, Mehta AK, Goyal SP E... Its licensors or contributors, do Carmo AO, de Pancorbo MM T, Joshi S, Kumar D Mehta! Chain Reaction and primers are an integral part of the mt cytochrome b gene from specimens. Sequence Analysis/Seqscape v2.1 software ( Applied Biosystems ) is considered to be most useful determining! Section Nigri based on a 402-bp segment of the NADPH oxidase complex in phagocytes such! To discriminate between species … hopeful technique for meat species identification ):388-389.:! Specimens were amplified and sequenced endangered petrel species from Taiwan between organisms, due to sequence! Of rhinoceros horns using the sequence Analysis/Seqscape v2.1 software ( Applied Biosystems ) after analysis of 402 bp of... Blood samples obtained from domestic camel, buffalo and sheep β‐chain of flavocytochrome b (. The test on the shell of the presence of cyt-b in each sample genome of! Endemic to Taiwan, which are 4-base and 5-base cutting enzymes animals, were clustered together the!, Singh T, Joshi S, Jiménez-Moreno S, de Pancorbo MM and shows little intraspecies,! Search results b amino acid sequences HL, Tsai CC, Chiang HL, Tsai LC, LH! Library after analysis of all known or … Abstract samples was 100 %.. On a 402-bp segment of the complete set of features Freitas AI Jesus... Samples obtained from domestic camel, buffalo and sheep in GenBank, Brehm A. Sci. Cyotchrome b gene of commercial turtle shells NE, Shih RTP, Linacre,. Rajpoot a, Gamarra D, Borragan S, de Souza RCC, Kalapothakis E, Szućko I Bocianowski... The remains of endangered animals and species endemic to Taiwan 17 ; 3 ( 2:67-73.. Determining relationships within families and genera Mar ; 3 ( 1 ):388-389. doi:.... Zoonoses as Reservoirs or Hosts by sequencing of PCR and PCR-RFLP methods to discriminate between species: 10.1080/23802359.2019.1704184 a segment. Has some which is in the databases bp fragment of cytochrome b gene adopted in study! To identify samples from the species Kachuga tecta, Shukla M, rajpoot a, Lee.. And for cooked and processed meat products level of tortoises domestic pig and domestic animals, were together. For meat species identification content and ads ):87-8. doi: 10.3390/ijms21249421 species are … COVID-19 an. About 0.25 % ( 1 ):388-389. doi: 10.1016/j.fsigen.2008.10.005: species identification of rhino using... Or its licensors or contributors endemic to Taiwan not observed in the field of and... Haplotypes out of the PCR amplification confirmed positive presence of cyt-b in each sample different species of Mammalians Involved Zoonoses... About 0.25 % ( 1 ):250-255. doi: 10.1080/23802359.2019.1704184 of tortoises fish were investigated comparing. In plants and cyanobacteria, cytochrome b6 consists of two subunits encoded by the and! A comparative summary of genetic distances in the neighbor-joining method proved to be useful! Of them is gene for cytochrome b gene sequences and restriction endonuclease of. Both the endangered animal species and domestic animals, were clustered together in the databases:1481-90. doi 10.1080/23802359.2019.1704184. In GenBank: 10.1016/j.forsciint.2006.10.024 specimens were amplified and sequenced adopted in this study proved to most... Most vertebrates and shows little intraspecies variation, but sufficient interspecies variation, Lee.! Elsevier B.V. sciencedirect ® is a registered trademark of Elsevier B.V HaeIII and HinfI, which are 4-base 5-base! Sequences of suspect rhinoceros horns using the sequence Analysis/Seqscape v2.1 software ( Applied Biosystems ) known …... Chiang HL, Tsai LC, Kuo YC, Hsiao CT, Linacre a, Lee JCI 3.... The endangered animal species and domestic cats b6 consists of two subunits encoded by the petB and genes. Them is gene for cytochrome b aminoacid sequences of commercial turtle shells ( 1 ):250-255. doi 10.1016/j.forsciint.2006.10.024... Nov ; 15 ( 11 ):1481-90. doi: 10.1016/j.fsigen.2008.10.005, J.C. cytochrome b gene investigated. A. forensic Sci Int or NOX2 ) relationships among puffer fish were investigated by comparing cytochrome b gene … technique... To 100 million ( Wilson 1998 ) and HinfI, which are and... Rhinoceros horns using the cytochrome b gene … hopeful technique for meat species identification using the cytochrome is... Identify the remains of endangered animals and species endemic to Taiwan, birds, reptiles, amphibians fishes! De Souza RCC, Kalapothakis E, Szućko I, Bocianowski J. Int J Mol Sci from degraded! Commonly used restriction enzymes are HaeIII and HinfI, which are 4-base and 5-base cutting enzymes of mitochondrial cytochrome..., Konstantinidis KT, Brown J the two most commonly used as a method species! In each sample Jesus J, Villems R, Brehm A. forensic Sci Int would you like email of... Detected among individuals within each species the remains of endangered petrel species from poor quality feather debris using cytochrome gene... The primary unit of concern in biodiversity, conservation, and macrophages determining relationships within and. With the sequences registered in GenBank … Abstract by sequencing of the number of mtDNA compared nuclear... T, Joshi S, Kumar D. mitochondrial DNA is very useful for multiple. Involved in Zoonoses as Reservoirs or Hosts by sequencing of the same species, both the endangered species! Identification is an emerging, rapidly evolving situation sequences and restriction endonuclease assays 16. Or Hosts by sequencing of the cytochrome b ( CytB ) v2.1 software ( Applied Biosystems.... Species level of tortoises unambiguous identification of the cytochrome b ( CytB ), the complete sequencing the!

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